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Sensitive method for measuring lactate dehydrogenase activity in human serum by differential-pulse polarography
S T Sulaiman1, T S al-Najafi, H S Hamdon
1Department of Chemistry, College of Science, University of Mosul, Iraq.
The Analyst
|October 1, 1994
Abstract:
A sensitive and accurate differential-pulse polarographic method is reported for the measurement of lactate dehydrogenase (LDH) activity. The method is based on the polarographic measurement of the NAD+ formed via the enzymic reaction of LDH with pyruvate and NADH. The method was successfully applied to measure LDH activity in the serum of normal and diseased individuals.