Related Experiment Videos
Correlation of bioenergetic parameters with cell death in Listeria monocytogenes cells exposed to nisin
K Winkowski1, M E Bruno, T J Montville
1Department of Food Science, New Jersey Agricultural Experiment Station, Cook College, Rutgers, State University of New Jersey, New Brunswick 08903.
Applied and Environmental Microbiology
|November 1, 1994
Abstract:
In Listeria monocytogenes, nisin induced ATP efflux, reduced the intracellular ATP concentration within 1 min, and dissipated the proton motive force within 2 min. Efflux accounted for only 20% of the ATP depletion, suggesting that ATP hydrolysis also occurred. ATP efflux depended on nisin concentration and followed saturation kinetics. These results suggest that nisin breaches the membrane permeability barrier in a manner more consistent with pore formation than with a nonspecific detergent-like membrane destabilization.