Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Erythrocyte metabolism

G J Brewer, W C Kruckeberg, C J Westover

    Progress in Clinical and Biological Research
    |January 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Red blood cell carbohydrate metabolism is vital for cell energy and oxygen release. Frozen storage preserves these functions, unlike liquid storage, but monitoring metabolic intermediates is key after thawing.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Treatment of age-related decreases in GTP levels restores endocytosis and autophagy.

    GeroScience·2025
    Same author

    Treatment of age-related decreases in GTP levels restores endocytosis and autophagy.

    bioRxiv : the preprint server for biology·2025
    Same author

    Behavioural responses of stable flies to cattle manure slurry associated odourants.

    Medical and veterinary entomology·2015
    Same author

    Chronic network stimulation enhances evoked action potentials.

    Journal of neural engineering·2010
    Same author

    Diagnosis of Wilson's disease.

    The Netherlands journal of medicine·2009
    Same author

    Neuron network activity scales exponentially with synapse density.

    Journal of neural engineering·2008
    Same journal

    DNA homology and chromosome stability: a sensitive yeast genetic system for identifying double-stranded DNA damage.

    Progress in clinical and biological research·2018
    Same journal

    Endotoxin and sepsis: molecular mechanisms of pathogenesis, host resistance, and therapy. Proceedings of the 4th Conference of the International Endotoxin Society. Nagoya, Japan, October 23-27, 1996.

    Progress in clinical and biological research·1998
    Same journal

    The pathogenic role of LBP in gram-negative sepsis and septic shock.

    Progress in clinical and biological research·1998
    Same journal

    The role of interleukin 6 in endotoxin-induced inflammatory responses.

    Progress in clinical and biological research·1998
    Same journal

    Role of nitric oxide and reactive oxygen species in endotoxin shock.

    Progress in clinical and biological research·1998
    Same journal

    Interaction of lipopolysaccharide with a mammalian lyso-phosphatidate acyltransferase (LPAAT) transfected into E. coli, and effect of lisofylline on LPAAT transfected into mammalian cells.

    Progress in clinical and biological research·1998
    See all related articles

    Area of Science:

    • Biochemistry
    • Hematology
    • Cellular Metabolism

    Background:

    • Red blood cell (RBC) carbohydrate metabolism is crucial for cellular energy (ATP) and oxygen release (2,3-DPG).
    • Traditional liquid storage methods impair these essential RBC functions.
    • Frozen storage offers long-term preservation of RBC viability and function.

    Purpose of the Study:

    • To investigate the impact of storage methods on RBC carbohydrate metabolism.
    • To evaluate the efficacy of frozen storage in maintaining RBC metabolic functions.
    • To establish glycolytic intermediate assays as a monitoring tool for RBC processing.

    Main Methods:

    • Analysis of red blood cell carbohydrate metabolism.
    • Comparison of liquid versus frozen storage techniques.

    Related Experiment Videos

  • Assay of glycolytic intermediates post-thaw and wash procedures.

    • Main Results:

    • Liquid storage compromises RBC ATP production and DPG levels.
    • Frozen storage effectively maintains RBC viability and metabolic functions.
    • Assays of glycolytic intermediates reveal metabolic status after thawing and washing.

    Conclusions:

    • RBC carbohydrate metabolism is essential for cell viability and oxygen transport.
    • Frozen storage is superior to liquid storage for preserving RBC metabolic integrity.
    • Monitoring glycolytic intermediates is a valuable method for assessing RBC quality after cryopreservation and processing.