[Development and testing of an indirect ELISA for the detection of antibodies against avian encephalomyelitis]

Because of the intensive efforts required and the dissatisfactory sensitivity achieved with the traditional serological diagnostic techniques for Avian Encephalomyelitis, an ELISA was developed and tested to show the presence of antibodies in chicken serum. Using a multi-phase antigen preparation and optimizing the reagents and serum dilutions, a method was developed which shows considerable advantages in sensitivity, specificity, practicability and costs compared with the Embryo Serum Neutralization Test.