Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cross-linking hemoglobin by design: lessons from using molecular clamps

R Kluger1, R T Jones, D T Shih

  • 1Department of Chemistry, University of Toronto, Ontario, Canada.

Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
|January 1, 1994
PubMed
Summary

Chemically modified hemoglobin, using acyl phosphate methyl esters, creates a cross-link in human hemoglobin. This cross-linked hemoglobin shows promise as a red cell substitute with suitable oxygen affinity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intravenous tranexamic acid and lower limb arthroplasty-a randomised controlled feasibility study.

Anaesthesia and intensive care·2018
Same author

Biocompatibility and immunogenicity of elastin-like recombinamer biomaterials in mouse models.

Journal of biomedical materials research. Part A·2017
Same author

Comparison of preoperative and intraoperative assessment of aortic stenosis severity by echocardiography.

British journal of anaesthesia·2017
Same author

Minimally invasive cardiac output monitoring: agreement of oesophageal Doppler, LiDCOrapid™ and Vigileo FloTrac™ monitors in non-cardiac surgery.

Anaesthesia and intensive care·2016
Same author

Measurement of peak aortic jet velocity in the perioperative period-machine variability: implications for assessment of aortic stenosis severity.

Anaesthesia and intensive care·2015
Same author

The relationship between superior vena cava diameter and collapsibility and central venous pressure.

Anaesthesia and intensive care·2015

Area of Science:

  • Biochemistry
  • Biomaterials Science
  • Chemical Engineering

Background:

  • Developing a safe and effective red blood cell substitute is crucial for transfusion medicine.
  • Chemical modification of hemoglobin offers a potential route to create such a substitute.

Purpose of the Study:

  • To systematically develop and characterize chemically modified hemoglobin as a red cell substitute.
  • To investigate the relationship between cross-link structure and oxygen binding properties.

Main Methods:

  • Design and synthesis of acyl phosphate methyl esters as anionic electrophiles for hemoglobin modification.
  • Kinetic studies to elucidate the reaction mechanism of acylation.
  • Introduction of cross-links in human hemoglobin using rigid dicarboxylic acid derivatives and trifunctional cross-linkers.

Related Experiment Videos

  • Analysis of oxygen binding properties of modified hemoglobin.

    • Main Results:

    • Acyl phosphate methyl esters selectively acylate amino groups in hemoglobin.
    • Rigid dicarboxylic acid derivatives create cross-links between specific beta subunits (Lys-82 and Val-1).
    • The 1-82 cross-link maintains cooperativity while reducing oxygen affinity, yielding suitable properties for a red cell substitute.
    • Trimesyl tris(methyl phosphate) efficiently produces hemoglobin with the desired 1-82 cross-link.

    Conclusions:

    • Chemically modified hemoglobin with a specific 1-82 beta subunit cross-link demonstrates suitable oxygen affinity for red cell substitute applications.
    • The developed chemical modification strategy is effective and yields materials comparable to those from recombinant technology.
    • Further research will focus on novel chemicals and structural insights for optimized hemoglobin-based oxygen carriers.