Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Convenient method to purify hemoglobin

H Sakai1, S Takeoka, H Nishide

  • 1Department of Polymer Chemistry, Waseda University, Tokyo, Japan.

Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analysis of radiotherapy in 1054 patients with primary central nervous system lymphoma treated from 1985 to 2009.

Clinical oncology (Royal College of Radiologists (Great Britain))·2014
Same author

Suppression of tumor metastasis by manganese superoxide dismutase is associated with reduced tumorigenicity and elevated fibronectin.

Oncology reports·2011
Same author

Spatial variation of gain and saturation in a fast axial flow CO(2) laser amplifier.

Applied optics·2010
Same author

Dispersive properties of a flexible KRS-5 fiber on magneto-optical effects at individual CO(2) laser lines: errata.

Optics letters·2009
Same author

Dispersive properties of a flexible KRS-5 fiber on magneto-optical effects at individual CO(2) laser lines.

Optics letters·2009
Same author

Haemoglobin-vesicles as artificial oxygen carriers: present situation and future visions.

Journal of internal medicine·2007

A new method purifies hemoglobin (Hb) from outdated red blood cells (RBCs) for blood substitutes. This process prevents methemoglobin (MetHb) formation, ensuring a pure, stable Hb solution suitable for large-scale production.

Area of Science:

  • Biochemistry
  • Biotechnology
  • Hematology

Background:

  • Hemoglobin-based blood substitutes require purified hemoglobin (Hb) as a starting material.
  • Outdated red blood cells (RBCs) are a potential source of Hb, but purification is challenging.
  • Methemoglobin (MetHb) formation is a common issue during Hb processing, compromising its function.

Purpose of the Study:

  • To establish a convenient and scalable method for purifying Hb from outdated RBCs.
  • To prevent MetHb formation during the purification process.
  • To ensure the purity and functional integrity of the Hb solution for blood substitute applications.

Main Methods:

  • Carbonylation of Hb within RBCs to prevent MetHb formation.
  • Hemolysis using organic solvent followed by centrifugation to remove stroma.

Related Experiment Videos

  • Heat treatment at 60°C to precipitate denatured proteins, followed by centrifugation.
  • Analysis of Hb purity and integrity using SDS-PAGE, Isoelectric Focusing (IEF), and oxygen binding assays.

    • Main Results:

    • A high purity Hb solution (25 g/dl) was successfully obtained from outdated RBCs.
    • The purification method effectively prevented MetHb formation.
    • SDS-PAGE and IEF confirmed the purity and lack of denaturation of the Hb.
    • Oxygen binding properties were preserved, indicating functional integrity.

    Conclusions:

    • A convenient and scalable method for purifying Hb from outdated RBCs has been developed.
    • The method yields a pure, stable Hb solution suitable for use in Hb-based blood substitutes.
    • This process addresses the challenge of MetHb formation and ensures the quality of the starting material.