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Related Experiment Videos

Structure and solution properties of lipidheme-microsphere

T Komatsu1, H Nishide, E Tsuchida

  • 1Department of Polymer Chemistry Waseda University, Tokyo, Japan.

Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
|January 1, 1994
PubMed
Summary

A novel synthetic artificial red cell, the lipidheme-microsphere (LH-M), demonstrates efficient oxygen binding and favorable physiological properties. This artificial red blood cell offers a promising alternative for oxygen transport applications.

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Biophysics

Background:

  • Development of artificial red blood cells is crucial for transfusion medicine.
  • Existing artificial oxygen carriers face challenges in mimicking natural red blood cell function.
  • Synthetic heme derivatives offer potential for oxygen binding capabilities.

Purpose of the Study:

  • To synthesize and characterize a novel totally synthetic artificial red cell, the lipidheme-microsphere (LH-M).
  • To evaluate the structural properties, solution characteristics, and oxygen binding ability of the LH-M.
  • To assess the physiological compatibility of the LH-M for potential therapeutic applications.

Main Methods:

  • Synthesis of lipidheme-microsphere (LH-M) using a phospholipid derivative of heme.

Related Experiment Videos

  • Electron microscopy for particle size determination (ca. 90 nm diameter).
  • Rheological, osmotic, and colloid osmotic pressure measurements.
  • Oxygen binding studies to determine P50(O2) at physiological conditions (pH 7.4, 37°C).

    • Main Results:

    • LH-M exhibits a particle diameter of approximately 90 nm.
    • LH-M suspension has a low viscosity (approx. 1.5 cP), with a mixed system (1:1 v/v) viscosity of 2.5 cP.
    • Physiological parameters such as specific gravity, osmotic pressure, and colloid osmotic pressure are within acceptable ranges.
    • Reversible oxygen binding demonstrated with P50(O2) of 41 torr.
    • LH-M shows higher oxygen solubility than human blood due to high heme concentration.

    Conclusions:

    • The lipidheme-microsphere (LH-M) represents a viable totally synthetic artificial red blood cell.
    • LH-M possesses favorable structural and solution properties, including low viscosity and physiological compatibility.
    • The demonstrated reversible oxygen binding and high oxygen solubility highlight its potential as an oxygen carrier.