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A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model
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Human carcinoma cell lines produce biologically active leukemia inhibitory factor (LIF)

H Kamohara1, K Sakamoto, T Ishiko

  • 1Department of Surgery II, Kumamoto University Medical School, Japan.

Research Communications in Molecular Pathology and Pharmacology
|August 1, 1994
PubMed

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Cell Lines01:16

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...