Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Photodynamic biologic tissue glue

J Khadem1, T Truong, J T Ernest

  • 1Department of Ophthalmology and Visual Science, University of Chicago, Illinois 60637.

Cornea
|September 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lacosamide concentration-dose (C/D) ratios during pregnancy: a case series.

European journal of clinical pharmacology·2025
Same author

Redressing inequities in women's cardiovascular health.

BMJ (Clinical research ed.)·2025
Same author

Ultrafiltration: Effect of process temperature (7°C and 50°C) on process performance and protein beverage physical, chemical, and sensory properties.

Journal of dairy science·2024
Same author

Achievement of Target Gain Larger than Unity in an Inertial Fusion Experiment.

Physical review letters·2024
Same author

Milk beverage base with lactose removed with ultrafiltration: Effect of fat and protein concentration on sensory and physical properties.

Journal of dairy science·2023
Same author

Lawson Criterion for Ignition Exceeded in an Inertial Fusion Experiment.

Physical review letters·2022

This study introduces a novel light-activated tissue glue for corneal repair. The developed laser-activated fibrinogen and riboflavin glue demonstrated effective sealing of corneal incisions, showing promise for ophthalmic surgery.

Area of Science:

  • Ophthalmology
  • Biomaterials Science
  • Surgical Innovation

Background:

  • Traditional heat-activated tissue glues can affect adjacent tissues.
  • Light-activated adhesives offer a potentially less damaging alternative for tissue repair.
  • Corneal wound closure requires strong and reliable adhesives.

Purpose of the Study:

  • To develop and evaluate a novel light-activated tissue glue for corneal repair.
  • To assess the efficacy of a fibrinogen and riboflavin-based glue activated by a blue-green laser.
  • To determine the tensile strength of corneal incisions sealed with the experimental glue.

Main Methods:

  • A tissue glue composed of 18% fibrinogen and 2.6 mg/ml riboflavin-5-phosphate was formulated.
  • The glue was activated using a blue-green (488-514 nm) Argon laser to cross-link corneal stromal collagen.

Related Experiment Videos

  • The adhesive strength was tested on 5-mm penetrating central corneal incisions in human cadaver eyes by measuring intraocular pressure until wound rupture.

    • Main Results:

    • The laser-activated tissue glue successfully sealed corneal incisions in all tested cadaver eyes.
    • The mean bursting pressure for the sealed corneal wounds was 154 mm Hg (range: 80-260 mm Hg).
    • The light-activated nature of the glue is hypothesized to minimize damage to surrounding tissues.

    Conclusions:

    • The developed laser-activated tissue glue is an effective adhesive for corneal tissue repair.
    • The study provides a strong foundation for further investigation into this novel ophthalmic adhesive.
    • Future research will focus on toxicity studies in living animal models.