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[Distribution of lysotypes and study of antibiotic sensitivity of Shigella flexneri 2 isolated in Iran]
Abstract:
152 strains of Shigella flexneri 2 isolated from the stools of patients having sporadic bacillary dysenteria have been studied for their phage types as well as for the presence of transferable resistance factor. The distribution of the phage types has shown a high predominance of lysotype 12 (67%). Proportion of Shigella flexneri 2 strains resistant to one or several antibiotics has been highest in the years 1968 and 1973. The most frequent transferable resistance pattern was found to be (Tc, Cm, Sm, Su) and the most successful transfer method was the celophane technique. Resistance loss varied according to the resistance pattern of the strains. Total loss of the resistance to Tc, Cm, Sm, Su occurred in 0,2-2 % only. Very often strains resistant to Tc, Cm, Sm, Su lost their resistance factor to Tc and Cm keeping the resistance factors for Su, Sm.
Insights
Shigella flexneri 2 strains show a high prevalence of lysotype 12 and antibiotic resistance, particularly tetracycline, chloramphenicol, streptomycin, and sulfadiazine. Transferable resistance factors were common, with limited overall resistance loss observed.
Area of Science:
- Microbiology
- Bacteriology
- Genetics
Context:
- Shigella flexneri is a significant cause of bacillary dysentery.
- Understanding antibiotic resistance patterns is crucial for effective treatment.
- The study focuses on Shigella flexneri serotype 2.
Purpose:
- To investigate the phage types of Shigella flexneri 2 strains.
- To determine the prevalence and patterns of transferable antibiotic resistance.
- To analyze the methods and extent of resistance loss.
Summary:
- 152 Shigella flexneri 2 strains from dysentery patients were analyzed.
- Lysotype 12 predominated (67%).
- Antibiotic resistance was highest in 1968 and 1973, with tetracycline, chloramphenicol, streptomycin, and sulfadiazine resistance being most frequent.
- The cellophane technique was most effective for transferring resistance.
- Total resistance loss was low (0.2-2%), often involving retention of streptomycin and sulfadiazine resistance.
Impact:
- Provides insights into the epidemiology of Shigella flexneri 2.
- Highlights the persistence of transferable antibiotic resistance.
- Informs public health strategies for managing dysentery outbreaks.