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[Determination of bacteriocinogenic types of strains of Proteus mirabilis]
H Józefowicz-Piatkowska1, K Piatkowski
1Zakład Mikrobiologii Lekarskiej AM w Lodzi.
Abstract:
The study was aimed at determination of bacterioenzymogenic activity of 351 Proteus strains (337 strains of Proteus mirabilis and 14 strains of Proteus Vulgaris) isolated in laboratory of Pediatric Institute of the Medical Academy in Lódź, mostly from urine (222 strains) and feces (99 strains). for determination of bacteriocinogenic properties, 15 index strains "S" were used. Of these, six were from collection of Senior (The University of Dundee Medical School, Scotland) and 9 from own Piatkowski collection (Lódź University). Detection of bacteriocinogenic strains and determination of their activity toward 15 index strains was performed by an own method (15). Out of total 551 strains tested, bacteriocinogenic properties were found in 263 (75%) of strains. Remaining 88 (25%) strains did not inhibit any of the 15 index strains. Sixty-two different types of bacteriocinogenicity were found. The results suggest that bacteriocinogenic typing of Proteus mirabilis strains permits for more precise characterization of properties of these bacteria useful for epidemiological purposes, than by application of other methods.
Insights
Bacteriocinogenic typing of Proteus strains, particularly Proteus mirabilis, offers a more precise method for characterizing these bacteria. This technique aids in epidemiological studies by identifying specific bacterial properties.
Area of Science:
- Microbiology
- Bacteriology
- Epidemiology
Background:
- Proteus strains are frequently isolated from clinical samples, particularly urine and feces.
- Understanding the properties of Proteus strains is crucial for epidemiological surveillance.
- Bacteriocinogenic activity is a key characteristic for bacterial strain differentiation.
Purpose of the Study:
- To determine the bacteriocinogenic activity of 351 Proteus strains (Proteus mirabilis and Proteus Vulgaris).
- To evaluate the potential of bacteriocinogenic typing for epidemiological characterization of Proteus strains.
Main Methods:
- Bacteriocinogenic properties were assessed using 15 index strains.
- An in-house method was employed to detect bacteriocinogenic strains and their activity.
- Strains were isolated primarily from urine and fecal samples.
Main Results:
- Bacteriocinogenic properties were detected in 263 (75%) of the tested Proteus strains.
- No inhibitory activity was observed in the remaining 88 (25%) strains.
- Sixty-two distinct types of bacteriocinogenicity were identified.
Conclusions:
- Bacteriocinogenic typing provides a more precise characterization of Proteus mirabilis strains compared to other methods.
- This typing method is valuable for epidemiological purposes in identifying and tracking bacterial strains.
- The study highlights the diversity of bacteriocinogenic activity within Proteus populations.