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Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance
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Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance

Published on: July 28, 2023

[Occurrence of Helicobacter pylori strains resistant to metronidazole]

E Andrzejewska1, J Jenek, H Klincewicz

  • 1Zakład Mikrobiologii Lekarskiej i Klinika Gastroenterologii AM im. Karola Marcinkowskiego w Poznaniu.

Medycyna Doswiadczalna I Mikrobiologia
|January 1, 1994
PubMed

Insights

High rates of metronidazole resistance in Helicobacter pylori strains were observed in patients with gastritis. This highlights the critical need for ongoing monitoring of antibiotic susceptibility in H. pylori infections.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Pharmacology

Background:

  • Helicobacter pylori (H. pylori) is a common cause of gastritis and peptic ulcers.
  • Metronidazole is a frequently used antibiotic for H. pylori eradication therapy.
  • Increasing antibiotic resistance poses a significant challenge to effective treatment.

Purpose of the Study:

  • To investigate the in vitro susceptibility of clinical Helicobacter pylori isolates to metronidazole.
  • To determine the prevalence of metronidazole resistance in H. pylori strains from patients with gastritis.

Main Methods:

  • Clinical strains of H. pylori were isolated from patients undergoing gastritis diagnosis.
  • Antibiotic susceptibility testing was performed using the disc diffusion method.
  • Isolates were analyzed for resistance patterns against metronidazole.

Main Results:

  • H. pylori was successfully isolated from 44 (67.7%) of the patients studied.
  • A high prevalence of metronidazole resistance was found, with 36 (81.8%) of the H. pylori strains exhibiting resistance.
  • Resistance rates were comparable between strains isolated from men (79.2%) and women (85%).

Conclusions:

  • The study reveals a significant level of metronidazole resistance in clinical H. pylori isolates.
  • Monitoring drug resistance patterns is crucial for guiding effective H. pylori eradication therapy.
  • These findings underscore the importance of antimicrobial stewardship in managing H. pylori infections.

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