[The rapid detection of arboviruses by dot immunoenzyme analysis on membrane filters]

A direct and an indirect variants of dot enzyme immunoassay on membrane filters (dot-EIA) for detection of arbovirus antigens and antibodies were developed. Both variants had similar sensitivity and specificity as compared with the solid-phase enzyme immunoassay usually used for this purpose. The advantages of dot-EIA consist in the possibility of specific detection of antigens in unpurified biological specimens by direct adsorption of the antigen on the membranes, economy aspects, and shortening of the time of analysis.