Related Experiment Videos
Localization of transglutaminase-reactive glutamine residues in bovine osteopontin
E S Sørensen1, L K Rasmussen, L Møller
1Protein Chemistry Laboratory, University of Aarhus, Denmark.
The Biochemical Journal
|November 15, 1994
Abstract:
Here we report the identification of two transglutaminase-reactive glutamines (Gln-34 and Gln-36) in bovine osteopontin (OPN). Sequence alignment revealed that these glutamines are conserved in all known OPN sequences, indicating a functional importance of this region of the protein. Furthermore, immunological analysis of bovine bone demonstrated that OPN is present in high-molecular-mass complexes in vivo. These findings support the functional aspects of a transglutaminase-catalysed cross-linking of OPN in facilitating cellular attachment and tissue calcification.