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Intracellular mediators of goblet cell degranulation in isolated pancreatic ducts
T J Wilkins1, J de Ondarza, S R Hootman
1Department of Physiology, Michigan State University, East Lansing 48824-1101.
Biochemical and Biophysical Research Communications
|November 30, 1994
Abstract:
The involvement of particular intracellular signalling pathways in agonist-evoked degranulation of guinea pig pancreatic duct goblet cells was investigated. Carbachol, vasoactive intestinal peptide (VIP), calcium ionophore A23187, phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA), cyclic AMP analogue Sp-5,6-DCl-cBIMPS and forskolin each caused degranulation of goblet cells in isolated ducts. Degranulation induced by carbachol was not inhibited by okadaic acid, cytochalasin-D or nocodazole. These results indicate that at least two major signalling pathways are involved in pancreatic duct goblet cell secretion.