Stimulation of cell-mediated low-density lipoprotein oxidative modification by oncostatin M

The effect of oncostatin M on low-density lipoprotein oxidative modification by human monocytes or murine endothelial cells was studied by determination of the lipid peroxidation products content and the electrophoretic mobility of the particle. In the range of concentrations from 10 to 30 ng/ml, oncostatin M induced a dose-dependent increase in LDL oxidation. The LDL degradation by J774 macrophage-like cells was also stimulated. Preincubation of cells with oncostatin M also enhanced the subsequent LDL modification. This effect was accompanied by a parallel increase in superoxide anion release by cells. Since oncostatin M is produced by immune cells, the described effect suggests a relationship between infection, inflammation and LDL oxidative modification.