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Molecular cloning of human cytosolic purine 5'-nucleotidase
J Oka1, A Matsumoto, Y Hosokawa
1National Institute of Health and Nutrition, Tokyo, Japan.
Biochemical and Biophysical Research Communications
|November 30, 1994
Abstract:
The cDNA of human cytosolic purine 5'-nucleotidase (EC 3.1.3.5), which is the supposed regulatory allosteric enzyme of purine nucleotide degradation, has been cloned from a human placenta cDNA library. The predicted open reading fram encodes a protein of 561 amino acids with a molecular mass of 64,966 Da. The deduced amino acid sequence exhibits 95% identity with the sequence of the B type subunit of chicken cytosolic purine 5'-nucleotidase. Northern blot analysis of human placenta poly(A) +RNA revealed a single band of 3.6 kilobases using the cloned cDNA.