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Chimerism in PCR products from a multigene family
D Bhavsar1, H Zheng, J Drysdale
1Department of Biochemistry, Tufts Medical School, Boston, MA 02111.
Biochemical and Biophysical Research Communications
|November 30, 1994
Abstract:
This paper describes a potential hazard in obtaining sequences of a multigene family by amplifying the mixed population by the polymerase chain reaction with primers from conserved sequences. We describe a product which appears to be a chimera formed during the PCR reaction from two different but related sequences.