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Charge-remote fragmentation of peptides derivatized with 4-aminonaphthalenesulphonic acid
I Lindh1, W J Griffiths, T Bergman
1Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
Rapid Communications in Mass Spectrometry : RCM
|October 1, 1994
Abstract:
A series of small peptides has been studied by negative-ion fast-atom bombardment mass spectrometry with collision-induced dissociation. It has been found that by derivatizing peptides with 4-aminonaphthalenesulphonic acid in a peptide linkage at the C-terminus, negative-ion formation can be enhanced and fragmentation in collision-induced dissociation reactions controlled. The peptide-naphthalenesulphonates show charge-remote fragmentations and the resultant spectra give sequence information.