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Updated: Aug 19, 2026

Microinjection of Medaka Embryos for use as a Model Genetic Organism
Published on: December 22, 2010
Establishment of a pluripotent cell line derived from a medaka (Oryzias latipes) blastula embryo
Y Wakamatsu1, K Ozato, T Sasado
1Laboratory of Freshwater Fish Stocks, Nagoya University, Japan.
Abstract:
A pluripotent cell line, OLES1, was established from a blastula embryo of a small freshwater fish, medaka (Oryzias latipes). Cells of this cell line were small and round, and they grew actively and stably in culture as dense clusters. They exhibited a positive alkaline phosphatase activity upon histochemical staining. When the cells were treated with retinoic acid, differentiation into various types of cells, including melanocytes, dopa-positive precursors of melanocytes, and cells with a molecular marker of skeletal muscles, troponin T, was induced in vitro. The present study opens a way to establishing embryonic stem cell lines in fish.
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