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Related Experiment Videos

African horse sickness virus structure

P Roy1, P P Mertens, I Casal

  • 1School of Public Health, University of Alabama at Birmingham 35294-0008.

Comparative Immunology, Microbiology and Infectious Diseases
|August 1, 1994
PubMed
Summary
This summary is machine-generated.

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African horse sickness virus (AHSV) shares structural similarities with bluetongue virus (BTV). Conserved gene segments are key for diagnostics, while variable segments influence serotype diversity.

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • African horse sickness virus (AHSV) is an Orbivirus within the Reoviridae family, with nine serotypes.
  • AHSV shares morphological and molecular similarities with bluetongue virus (BTV), another Orbivirus.
  • AHSV possesses a double-layered capsid composed of seven structural proteins (VP1-7) and a genome of 10 double-stranded RNA segments.

Purpose of the Study:

  • To review the properties of African horse sickness virus genes and their encoded products.
  • To analyze the conservation and variability of AHSV gene segments across different serotypes.
  • To explore the phylogenetic relationships between AHSV, BTV, and EHDV.

Main Methods:

  • Sequence and hybridization analyses of AHSV dsRNA segments.

Related Experiment Videos

  • Comparison of nucleotide and predicted amino acid sequences of viral genes and proteins.
  • Expression of AHSV capsid proteins using recombinant baculoviruses.

    • Main Results:

    • Core protein genes (VP3, VP7) and non-structural protein genes (NS1, NS2) are highly conserved among AHSV serotypes.
    • Genes encoding outer capsid proteins (VP2, VP5) and NS3/NS3A proteins show greater variability.
    • Phylogenetic analysis reveals a close relationship between AHSV, BTV, and EHDV.
    • Expressed AHSV capsid proteins are biochemically and antigenically similar to authentic proteins.

    Conclusions:

    • The VP7 protein, being highly conserved, is a valuable diagnostic reagent for AHSV.
    • Variability in outer capsid and NS3/NS3A genes contributes to AHSV serotype diversity.
    • Understanding AHSV gene and protein properties aids in diagnostics and phylogenetic studies.