[Histological technique for intramural ganglia in thick tissue preparation (author's transl)]

A simple staining technique for gastrointestinal tract neurons in Auerbach's plexus is proposed in thick tissue preparation. The ganglion cells between two muscular layers are easily seen by optical microscope examination in "spread" preparations up to 300 micron in thickness, following dehidratation. Routine paraffin sections can be used. Neurons in thicker preparations (over 300 micron) like in human small intestine can also be seen but only by using small to medium magnifications. A special fixation procedure was developed for this technique.