Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Early thymic regeneration after irradiation

G G Fredrickson1, R S Basch

  • 1Kaplan Cancer Center, New York, NY 10016.

Developmental and Comparative Immunology
|May 1, 1994
PubMed
Summary

Whole body irradiation causes thymic atrophy, but regeneration begins with radioresistant precursors. Early thymic regeneration involves mature T cells and early progenitors expressing T-cell lineage markers.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immortalized multipotential mesenchymal cells and the hematopoietic microenvironment.

Journal of hematotherapy & stem cell research·2001
Same author

mVH1, a dual-specificity phosphatase whose expression is cell cycle regulated.

Mammalian genome : official journal of the International Mammalian Genome Society·2000
Same author

Identification of four human cDNAs that are differentially expressed by early hematopoietic progenitors.

Experimental hematology·2000
Same author

Hematopoietic progenitor cells grow on 3T3 fibroblast monolayers that overexpress growth arrest-specific gene-6 (GAS6).

Proceedings of the National Academy of Sciences of the United States of America·2000
Same author

Expression of CD41 and c-mpl does not indicate commitment to the megakaryocyte lineage during haemopoietic development.

British journal of haematology·1999
Same author

Complementary and antagonistic effects of IL-3 in the early development of human megakaryocytes in culture.

British journal of haematology·1998

Area of Science:

  • Immunology
  • Cell Biology
  • Developmental Biology

Background:

  • Whole body irradiation leads to significant thymic atrophy.
  • Thymic regeneration after sublethal irradiation is rapid, initiated by radioresistant intrathymic precursors.

Purpose of the Study:

  • To analyze the early cellular events during thymic regeneration after irradiation.
  • To characterize the phenotypes of cells involved in thymic recovery using T-cell lineage markers.

Main Methods:

  • Utilized monoclonal antibodies against various T-lineage differentiation markers.
  • Employed three-color flow microfluorometric analysis to assess cell populations.
  • Investigated thymic cellularity and phenotypes at early time points post-irradiation.

Main Results:

  • The majority of early regenerative cells exhibit mature T-cell phenotypes (CD4-/CD8-, CD4+/CD8-, CD4-/CD8+).
  • A decline in mature T-cell phenotypes occurs between days 6 and 12.
  • Early populations also include CD3- cells (CD4+ or CD8+ HSA+), resembling intrathymic precursors, and Thy-1 negative T-cell lineage-expressing cells.

Conclusions:

  • Early thymic regeneration involves a mix of mature T cells and progenitor populations.
  • Intrathymic precursors and Thy-1 negative T cells are key components of the initial regenerative response.
  • Understanding these early cellular dynamics is crucial for comprehending thymic recovery post-irradiation.

Related Experiment Videos