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Updated: Aug 14, 2026

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format
Published on: September 17, 2016
SPO13 negatively regulates the progression of mitotic and meiotic nuclear division in Saccharomyces cerevisiae
1Department of Radiation and Cellular Oncology, University of Chicago, Illinois 60637.
Abstract:
The meiosis-specific yeast gene SPO13 has been previously shown to be required to obtain two successive divisions in meiosis. We report here that vegetative expression of this gene causes a CDC28-dependent cell-cycle arrest at mitosis. Overexpression of SPO13 during meiosis causes a transient block to completion of the meiosis I division and suppresses the inability of cdc28ts strains to execute meiosis II. The spo13 defect can be partially suppressed by conditions that slow progression of the first meiotic division. Based on the results presented below, we propose that SPO13 acts as a meiotic timing function by transiently blocking progression through the meiosis I division, thereby allowing (1) coordination of the first division with assembly of the reductional segregation apparatus, and (2) subsequent entry into a second round of segregation to separate replicated sister chromatids without an intervening S-phase.
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