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Urinary trypsin inhibitor has a protective effect on the amnion
E el Maradny1, N Kanayama, A Halim
1Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Japan.
Gynecologic and Obstetric Investigation
|January 1, 1994
Summary
Urinary trypsin inhibitor-related substance (UTI-R) is significantly higher in neonatal urine than in adults. UTI may protect the amnion from inflammatory factors like IL-1 beta and TNF-beta.
Area of Science:
- Reproductive Biology
- Biochemistry
- Immunology
Background:
- Urinary trypsin inhibitor-related substance (UTI-R) is a key protein with potential roles in biological processes.
- Understanding UTI-R levels in different physiological states, such as neonatal development, is crucial for assessing its function.
Purpose of the Study:
- To quantify UTI-R in various biological samples including amniotic fluid, neonatal urine, meconium, and adult urine.
- To investigate the presence of UTI in placental tissues.
- To examine the protective effect of UTI on amnion cells against inflammatory cytokines.
Main Methods:
- Measurement of UTI-R concentrations using established assays.
- Immunostaining techniques to detect UTI in placental tissues.
- Fura-2 acetoxymethyl ester assay to assess amnion cell response to inflammatory stimuli (IL-1 beta, TNF-beta, endotoxin) with and without UTI incubation.
Main Results:
- Neonatal urine showed significantly higher UTI-R concentrations compared to adult urine and meconium.
- UTI was immunolocalized in the amnion, umbilical cord, and placenta.
- Interleukin-1 beta and tumor necrosis factor-beta significantly increased fura-2 release from amnion cells, an effect largely abolished by UTI pre-incubation.
Conclusions:
- Neonatal urinary excretion of UTI-R is notably higher than in adults, suggesting developmental differences.
- UTI is present in key pregnancy-related tissues.
- UTI demonstrates a protective role for amnion cells against inflammatory insults, particularly IL-1 beta and TNF-beta.