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DNA topoisomerase I from Mycobacterium smegmatis
1Centre for Genetic Engineering, Indian Institute of Science, Bangalore.
Indian Journal of Biochemistry & Biophysics
|August 1, 1994
Abstract:
DNA topoisomerase I has been purified from Mycobacterium smegmatis to near homogeneity using different column chromatographic techniques. The enzyme activity relaxes form I DNA into form IV DNA, requiring Mg2+, but not ATP or any other cofactors for its activity. Several properties of the enzyme were found to be similar to that of the prototype enzyme, Escherichia coli topoisomerase I.