Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Disk diffusion susceptibility testing of dermatophytes with allylamines

P V Venugopal1, T V Venugopal

  • 1Institute of Microbiology, Madurai, Medical College, India.

International Journal of Dermatology
|October 1, 1994
PubMed
Summary

Allylamines like naftifine and terbinafine are effective for antifungal susceptibility testing. Disk diffusion is a reliable method for determining the best antifungal drug therapy for dermatophytes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of Azadirachta indica (neem) on the growth pattern of dermatophytes.

Indian journal of medical microbiology·2007
Same author

In vitro susceptibility of filamentous fungi to itraconazole.

Indian journal of pathology & microbiology·1995
Same author

Disk diffusion susceptibility testing of dermatophytes with imidazoles.

Indian journal of pathology & microbiology·1995
Same author

Pale grain eumycetomas in Madras.

The Australasian journal of dermatology·1995
Same author

Antimycotic susceptibility testing of mould-fungi with allylamines by disk diffusion.

Indian journal of medical sciences·1995
Same author

Antidermatophytic activity of garlic (Allium sativum) in vitro.

International journal of dermatology·1995

Area of Science:

  • Mycology
  • Pharmacology
  • Clinical Microbiology

Background:

  • Allylamines represent a novel class of antifungal agents with broad-spectrum efficacy.
  • Accurate antifungal susceptibility testing is crucial for selecting effective treatments, especially with emerging antifungal drugs.

Purpose of the Study:

  • To evaluate the in vitro susceptibility of clinical dermatophyte isolates to naftifine and terbinafine.
  • To assess the utility of agar dilution and disk diffusion methods for antifungal susceptibility testing.

Main Methods:

  • Susceptibility testing of 43 clinical dermatophyte isolates (Microsporum, Trichophyton, Epidermophyton floccosum) against naftifine and terbinafine.
  • Utilized agar dilution and disk diffusion techniques for in vitro evaluation.

Related Experiment Videos

Main Results:

  • Terbinafine demonstrated higher activity with minimal inhibitory concentration (MIC) values ranging from <= 0.0001-0.1 µg/mL.
  • Naftifine showed MIC values in the range of 0.001-0.5 µg/mL.
  • Both drugs exhibited good correlation between MICs and disk inhibition zones, with significant correlation coefficients (P < 0.001).

Conclusions:

  • Naftifine and terbinafine are suitable for antifungal susceptibility testing of dermatophytes using the disk diffusion method.
  • Standardized disk diffusion testing holds promise as a valuable laboratory procedure for filamentous fungi to guide antifungal therapy.