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Molecular cloning and characterization of murine cyclin E
1Department of Pathology and Cell Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107.
Biochemical and Biophysical Research Communications
|June 15, 1994
Summary
Researchers identified the murine cyclin E gene (cycE) using a monoclonal antibody. This gene is crucial for development, showing specific expression patterns in fetal and adult mouse tissues.
Area of Science:
- Molecular Biology
- Developmental Biology
- Genetics
Background:
- Cyclins are key regulators of the cell cycle.
- Understanding cyclin expression is vital for studying development and disease.
- Murine cyclin E (cycE) function and expression patterns were previously unclear.
Purpose of the Study:
- To identify and characterize the gene encoding murine cyclin E.
- To investigate the expression patterns of murine cyclin E during development and in adult tissues.
Main Methods:
- Monoclonal antibody development against mouse E6496D cell lysate.
- cDNA analysis to identify the cyclin E gene.
- RNA detection (e.g., Northern blot or similar) to assess gene expression.
Main Results:
- The murine cyclin E gene was identified, featuring a cyclin box and a long 3' UTR.
- Murine cyclin E shares high homology (>75%) with human cyclin E.
- cycE mRNA is expressed in fetal tissues, embryonal carcinoma F9, and yolk sac PYS-2 cells, but not in preimplantation stages.
- In adult mice, cycE mRNA is detectable in spleen, testis, and brain.
Conclusions:
- Murine cyclin E is developmentally regulated.
- The expression of cycE is uneven across different fetal and adult mouse tissues.
- This study provides foundational data on murine cyclin E's role in development.