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Identification by 2D-page analysis of salt-stress induced proteins in radish (Raphanus sativus)

F Lopez1, G Vansuyt, J Derancourt

  • 1Laboratoire de Biochimie et Physiologie Végétales, CNRS URA 573, ENSAM-INRA, Montpellier, France.

Cellular and Molecular Biology (Noisy-Le-Grand, France)
|February 1, 1994
PubMed

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SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...