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C-CAM expression in the developing rat central nervous system

H Sawa1, K Kamada, H Sato

  • 1Department of Neurosurgery, Kyorin University, School of Medicine, Tokyo, Japan.

Brain Research. Developmental Brain Research
|March 18, 1994
PubMed
Summary
This summary is machine-generated.

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Cell-CAM (cell adhesion molecule) is expressed in developing rat brain microvessels, not neurons. This immunoglobulin superfamily member may mediate adhesion between endothelial cells, pericytes, and astrocytes, aiding vessel wall maturation.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Cellular adhesion molecule (C-CAM) is a transmembrane glycoprotein in the immunoglobulin superfamily.
  • C-CAM mediates homophilic, calcium-independent intercellular adhesion.
  • Expressed in adult rat epithelia, endothelia, and hematopoietic cells.

Purpose of the Study:

  • To investigate the expression and role of C-CAM in the developing rat central nervous system.
  • To determine C-CAM's involvement in microvessel development and cell interactions.

Main Methods:

  • Molecular cloning and sequence analysis to identify C-CAM isoforms.
  • Immunohistochemistry and immunoelectron microscopy to localize C-CAM.
  • In situ hybridization to confirm expression in microvessel walls.

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Main Results:

  • C-CAM expression was not observed in neurons.
  • C-CAM expression initiated in microvessels around embryonic day E-13, peaking by E-15.
  • Localization to abluminal endothelial cells and pericytes, suggesting heterotypic adhesion roles.
  • Expression extended to astrocytes during the perinatal period.

Conclusions:

  • C-CAM plays a role in the developing rat central nervous system, specifically in microvessels.
  • C-CAM is involved in heterotypic adhesion between endothelial cells, pericytes, and astrocytes.
  • C-CAM likely contributes to the maturation of the vessel walls.