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Biochemical markers for assessing skeletal growth

S P Robins1

  • 1Biochemical Sciences Division, Rowett Research Institute, Bucksburn, Aberdeen, Scotland, UK.

European Journal of Clinical Nutrition
|February 1, 1994
PubMed
Summary

Pyridinium crosslinks are superior markers for collagen degradation and bone resorption compared to hydroxyproline. Combining multiple skeletal markers offers a comprehensive assessment of bone turnover and balance.

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Urinary excretion of pyridinium crosslinks in short children treated with growth hormone.

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Area of Science:

  • Biochemistry
  • Orthopedics
  • Metabolic Bone Disease

Background:

  • Skeletal turnover assessment relies on collagen metabolism markers.
  • Hydroxyproline and hydroxylysine glycosides are collagen degradation markers but have limitations.
  • Pyridinium crosslinks offer more specific and sensitive collagen degradation markers.

Purpose of the Study:

  • To evaluate biochemical markers for skeletal turnover.
  • To compare the efficacy of different collagen degradation markers.
  • To assess markers for bone formation and cartilage metabolism.

Main Methods:

  • HPLC for urinary crosslink quantification.
  • Development of direct immunoassay for urinary crosslinks.
  • Measurement of serum C-terminal propeptide of procollagen type I (PICP) and osteocalcin.
  • Analysis of crosslink excretion in relation to growth velocity in children.

Main Results:

  • Pyridinium crosslinks are specific, sensitive, diet-independent markers of bone resorption.
  • Urinary crosslink excretion correlates with growth velocity in children.
  • Serum PICP and osteocalcin are potential bone formation markers but require further validation.
  • Current markers for cartilage metabolism are insufficient.

Conclusions:

  • Pyridinium crosslinks are validated indices of bone resorption.
  • A combination of markers is recommended for comprehensive skeletal metabolism assessment.
  • Further development of cartilage metabolism markers is needed.

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