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Air samples from a building with asbestos-containing material: asbestos content and in vitro toxicity on rat pleural
H Dong1, L Saint-Etienne, A Renier
1Laboratory of Environmental Cellular and Molecular Pathology, Institut National de la Santé et de la Recherche Médicale (INSERM) U. 139, CHU Henri Mondor, Créteil, France.
Abstract:
Buildings equipped with asbestos-containing material may release asbestos fibers, the toxicity of which remains uncertain because of the generally low dose level. In 22 air samples collected in a building the asbestos level ranged between 0 and 0.027 f/ml. Both chrysotile and amphiboles were found. There was no association between in vitro cytotoxicity on rat pleural mesothelial cells and asbestos content, but there was a significant correlation with the total amount of particulate material. Four samples exhibited an enhancement of DNA synthesis in cells arrested in G1 with 5 mM hydroxyurea. This is more likely related to the particulate matter associated with asbestos fibers.