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Comparison of polyacrylamide gel electrophoresis & silver staining method with ELISA for rotavirus detection
1Department of Microbiology, Maulana Azad Medical College, New Delhi.
The Indian Journal of Medical Research
|February 1, 1994
Abstract:
A total of 421 human fecal samples were examined for the presence of rotavirus by enzyme-linked immunosorbent assay (ELISA) and by a modified polyacrylamide gel electrophoretic (PAGE) analysis of viral genome. Similar results by both methods were seen in 412 (97.86%) samples. The proportion of ELISA + PAGE - samples (2/421) was lower than the proportion of ELISA - PAGE + samples (7/421). This difference was not significant. The modified PAGE technique for the detection of viral RNA was found to be a rapid, simple, reliable and less expensive technique.