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Strip ELISA for the detection of IgG antibodies to Toxoplasma gondii
1Laboratory of Parasitology & Immunodiagnostics, Research & Development, Hindustan Antibiotics Ltd., Pune.
An enzyme linked immunosorbent assay (ELISA) has been developed using a polycarbonate coated strip and penicillinase for the detection of IgG antibodies to T. gondii. Of 40 serum samples from patients clinically suspected to have toxoplasma infection, 24 were positive for IgG antibodies to T. gondii. Strip ELISA showed 95.83 per cent sensitivity and 89.4 per cent specificity. This method is simple (end result assessed visually), sensitive (detection of antitoxoplasma IgG antibodies to a level of 15 IU/ml) and reproducible (5.9 and 11.6 intra and inter percentage of coefficient of variation respectively).
An enzyme linked immunosorbent assay (ELISA) has been developed using a polycarbonate coated strip and penicillinase for the detection of IgG antibodies to T. gondii. Of 40 serum samples from patients clinically suspected to have toxoplasma infection, 24 were positive for IgG antibodies to T. gondii. Strip ELISA showed 95.83 per cent sensitivity and 89.4 per cent specificity. This method is simple (end result assessed visually), sensitive (detection of antitoxoplasma IgG antibodies to a level of 15 IU/ml) and reproducible (5.9 and 11.6 intra and inter percentage of coefficient of variation respectively).