Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hypoxic vasodilation does not require nitric oxide (EDRF/NO) synthesis

B Vallet1, S E Curtis, M J Winn

  • 1Department of Physiology and Biophysics, University of Alabama at Birmingham 35294.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|March 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Live predator stress in adolescence results in distinct adult behavioral consequences and dorsal diencephalic brain activation patterns.

Behavioural brain research·2020
Same author

Postoperative continuous non-invasive cardiac output monitoring on the ward: a feasibility study.

Journal of clinical monitoring and computing·2020
Same author

When ethics collides with a legal gap in emergency life-threatening conditions.

British journal of anaesthesia·2018
Same author

Increased exposure of plankton to arsenic in contaminated weakly-stratified lakes.

The Science of the total environment·2018
Same author

Neurokinin-1 receptor antagonism attenuates neuronal activity triggered by stress-induced reinstatement of alcohol seeking.

Neuropharmacology·2015
Same author

Thermomicrobium carboxidum sp. nov., and Thermorudis peleae gen. nov., sp. nov., carbon monoxide-oxidizing bacteria isolated from geothermally heated biofilms.

International journal of systematic and evolutionary microbiology·2014
Same journal

Thoroughbred horses susceptible to Recurrent Exertional Rhabdomyolysis have elevated skeletal muscle mitochondrial capacities.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
Same journal

Change in Neutrophil-to-Lymphocyte Ratio after acute and chronic exercise: A Systematic Review and Meta-Analysis.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
Same journal

Ankylosing spondylitis and muscle sympathetic nerve activity: a case study.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
Same journal

Intracranial vasomotor and blood flow responses to light intensity aerobic exercise in young adults: a 4D flow MRI study.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
Same journal

Comparative assessments of the COSMED adaptive mixing chamber vs. breath-by-breath methods for oxygen uptake measurements in recreationally active adults.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
Same journal

Can we assess exercise metabolism from skin? Metabolomic profiles in skin dialysate collected during exercise.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
See all related articles

Inhibition of nitric oxide (NO) production did not prevent vasodilation during hypoxia but did increase vascular resistance. Oxygen extraction remained unaffected by NO inhibition in canine hindlimbs.

Area of Science:

  • Cardiovascular Physiology
  • Respiratory Physiology
  • Biochemistry

Background:

  • Nitric oxide (NO), also known as endothelium-derived relaxing factor, plays a crucial role in regulating vascular tone.
  • Understanding NO's role in hypoxic vasodilation and oxygen extraction is vital for comprehending circulatory responses to low oxygen conditions.

Purpose of the Study:

  • To investigate the effect of inhibiting nitric oxide production on hypoxic vasodilation and oxygen (O2) extraction in an in situ canine hindlimb model.
  • To determine if nitric oxide synthase inhibition interferes with the O2 extraction capacity during ischemic (IH) or hypoxic hypoxia (HH).

Main Methods:

  • Inhibition of nitric oxide production using NG-nitro-L-arginine methyl ester (L-NAME) in canine hindlimbs.
  • Induction of ischemic hypoxia (IH) and hypoxic hypoxia (HH) while monitoring hindlimb vascular resistance (LVR) and O2 uptake.

Related Experiment Videos

  • Comparison of physiological parameters between control and L-NAME treated groups under hypoxic conditions.

    • Main Results:

    • L-NAME administration increased baseline hindlimb vascular resistance and O2 uptake.
    • Inhibition of NO production did not prevent vasodilation in response to hypoxic hypoxia (HH) or ischemic hypoxia (IH).
    • Oxygen extraction ratio and O2 uptake remained similar between control and L-NAME treated groups during hypoxia.

    Conclusions:

    • Nitric oxide production is not essential for mediating vasodilation during hypoxic conditions in the canine hindlimb.
    • Inhibition of nitric oxide synthesis does not impair the ability of the hindlimb to extract oxygen during periods of reduced oxygen availability.