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Related Experiment Videos

Structural determination and packing analysis of a cholesteryl caprate/cholesteryl laurate solid solution

M P McCourt1, P Strong, W Pangborn

  • 1Electron Diffraction Department, Medical Foundation of Buffalo, Inc., NY 14203.

Journal of Lipid Research
|April 1, 1994
PubMed
Summary

This study reveals that cholesteryl decanoate and cholesteryl laurate form a solid solution with a unique lamellar packing. This packing suggests micro-fractionation of acyl chains, indicating non-ideal phase behavior in the crystal structure.

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X-ray Diffraction: A Practical Approach.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2008

Area of Science:

  • Crystallography
  • Materials Science
  • Biochemistry

Background:

  • Cholesteryl ester solid solutions exhibit complex phase behavior.
  • Understanding the crystal structure of these solutions is crucial for predicting their properties.

Purpose of the Study:

  • To determine the X-ray crystal structure of a cholesteryl decanoate/cholesteryl laurate solid solution.
  • To elucidate the packing arrangement and acyl chain distribution within the solid solution.

Main Methods:

  • X-ray crystal structure analysis of a bulk-grown solid solution (molar ratio 0.56/0.43).
  • Unit cell determination (monoclinic, P2(1) space group) and refinement (R factor 0.138).

Main Results:

  • The crystal structure exhibits a lamellar packing with characteristics suggesting micro-fractionation of acyl chains.

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  • Cell constants approximate Vegard's law, indicating near-ideal mixing at a macroscopic level.
  • Non-ideal phase behavior is linked to partitioned acyl chain distribution, contrasting with random co-packing in ideal solutions.

    • Conclusions:

    • The solid solution displays non-ideal phase behavior attributed to micro-fractionation of acyl chains.
    • This partitioning contrasts with the random co-packing observed in more ideal cholesteryl ester solid solutions.
    • Partial occupancy of atomic sites at chain termini is suggested due to non-stoichiometric component combinations.