Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Microencapsulation of Lactococcus lactis subsp. cremoris

B C Larisch¹, D Poncelet, C P Champagne

¹Department of Chemical Engineering, McGill University, Montréal, Québec, Canada.

Journal of Microencapsulation

|March 1, 1994

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Inhibition of the Psychrotrophic Bacteria of Raw Milk by Addition of Lactic Acid Bacteria.

Journal of food protection·2019

Same author

Complexity in mood disorder diagnosis: fMRI connectivity networks predicted medication-class of response in complex patients.

Acta psychiatrica Scandinavica·2018

Same author

Cell Microencapsulation: Dripping Methods.

Methods in molecular biology (Clifton, N.J.)·2016

Same author

Impact of a yogurt matrix and cell microencapsulation on the survival of Lactobacillus reuteri in three in vitro gastric digestion procedures.

Beneficial microbes·2015

Same author

Therapeutic cell encapsulation techniques and applications in diabetes.

Advanced drug delivery reviews·2013

Same author

Development of formulations to improve the controlled-release of linalool to be applied as an insecticide.

Journal of agricultural and food chemistry·2012

Same journal

Rationally engineered essential oil-loaded nanocarriers for acne vulgaris: integrating multiscale molecular modeling, machine learning, and response surface optimization.

Journal of microencapsulation·2026

Same journal

Retinyl palmitate-loaded nanostructured lipid carriers prepared by the phase inversion temperature method: Physicochemical properties, <i>in vitro</i> skin permeation, and occlusion ability.

Journal of microencapsulation·2026

Same journal

Green synthesis of silver nanoparticles using <i>Swertia chirayita</i> and their antioxidant and anticancer potential.

Journal of microencapsulation·2026

Same journal

Management of coronary artery disease via simvastatin-loaded novasomes.

Journal of microencapsulation·2026

Same journal

Phyto-engineered CuO nanoparticles from gum <i>Eucalyptus camaldulensis</i>: a GC-MS, molecular docking, and bioactivity study.

Journal of microencapsulation·2026

Same journal

Development and optimization of gallic acid-enriched nanostructured lipid carriers for the amelioration of rheumatic inflammation: <i>in-vitro</i> and <i>in-vivo</i> study.

Journal of microencapsulation·2026

See all related articles

Microencapsulation of Lactococcus lactis using alginate/poly-L-lysine (alg/PLL) membranes yields viable cells. While slightly slower than free cells, encapsulated bacteria show promising activity for milk acidification.

Area of Science:

Food Microbiology
Biotechnology
Biomaterials Science

Background:

Lactococcus lactis subsp. cremoris is crucial for dairy fermentation.
Microencapsulation techniques aim to improve microbial stability and functionality.
Evaluating different membrane materials is essential for successful encapsulation.

Purpose of the Study:

To assess the viability and activity of Lactococcus lactis subsp. cremoris microencapsulated in alginate/poly-L-lysine (alg/PLL), nylon, and crosslinked polyethyleneimine (PEI) membranes.
To compare the milk acidification performance of encapsulated cells with free cells.
To determine the lactic acid production rate of encapsulated Lactococcus lactis.

Main Methods:

Microencapsulation of Lactococcus lactis subsp. cremoris using alginate/poly-L-lysine, nylon, and crosslinked polyethyleneimine membranes.

Related Experiment Videos

Assessment of cell viability and activity post-encapsulation.

Measurement of milk pH reduction rates and lactic acid production by encapsulated and free cells.

Main Results:

Nylon and PEI encapsulation methods exhibited toxic effects.
Alg/PLL encapsulation maintained cell viability and activity, with acidification rates proportional to cell concentration.
Encapsulated cells (4 x 10^8 cfu/ml) acidified milk to pH 5.5 17% slower than free cells.
Lactic acid production rate was approximately 2 mmol/h at 4 x 10^8 cfu/ml encapsulated cell concentration.

Conclusions:

Alginate/poly-L-lysine (alg/PLL) microencapsulation is a viable method for Lactococcus lactis subsp. cremoris.
Encapsulated cells demonstrate functional activity in milk acidification, though at a reduced rate compared to free cells.
Higher encapsulated cell concentrations may achieve comparable activity to free cells.