Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Platelet-activating factor and WEB-2086 directly modulate rat cardiomyocyte contractility

L M Delbridge1, A G Stewart, C M Goulter

  • 1Department of Physiology, University of Melbourne, Parkville, Victoria, Australia.

Journal of Molecular and Cellular Cardiology
|February 1, 1994
PubMed
Summary

Platelet-activating factor (PAF) directly reduces adult cardiomyocyte contractility via receptor-mediated pathways. A PAF antagonist, WEB-2086, blocks this effect and enhances contractility independently.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Emerging Frontiers in Clinical Mycology: Innovations, Insights, and Impacts.

Mycoses·2026
Same author

A natural experiment during lockdown and on-going care-home COVID-19 outbreaks showed a single dose of vaccine reduced hospitalisation and deaths of care-home residents in North West England.

Public health·2024
Same author

Environmental Public Health practice: designing and delivering a locally desirable service.

Public health·2023
Same author

Effects of Post-Anthesis High-Temperature Stress on Carbon Partitioning and Starch Biosynthesis in a Spring Wheat (Triticum aestivum L.) Adapted to Moderate Growth Temperatures.

Plant & cell physiology·2023
Same author

APICAL INTRUSIVE GROWTH OF FIBRES FROM THE FLOWERING STEM OF LOLIUM TEMULENTUM L.

The New phytologist·2022
Same author

A mathematical model for simulating the spread of a disease through a country divided into geographical regions with different population densities.

Journal of mathematical biology·2022

Area of Science:

  • Cardiology
  • Pharmacology
  • Cell Physiology

Background:

  • Platelet-activating factor (PAF) effects on cardiac contractility are complex and debated.
  • Multicellular preparations obscure direct PAF actions on cardiomyocytes due to secondary mediator release.

Purpose of the Study:

  • To investigate the direct inotropic effects of PAF on isolated adult rat cardiomyocytes.
  • To characterize the actions of a specific PAF receptor antagonist, WEB-2086, on cardiomyocyte contractility.

Main Methods:

  • Enzymatically isolated adult rat cardiomyocytes were treated with varying concentrations of PAF (10^-11 to 10^-7 M).
  • The effects of PAF and the antagonist WEB-2086 (10^-5 M) on cell shortening and contraction parameters were measured.
  • Co-treatment experiments with PAF and WEB-2086 were performed.

Related Experiment Videos

Main Results:

  • PAF (10^-7 M) significantly reduced maximum cell shortening by 23% and delayed excitation-contraction coupling.
  • Lower PAF concentrations induced smaller negative inotropic effects; no positive inotropic effect was observed.
  • WEB-2086 alone enhanced maximum cell shortening by 16% and prolonged the contraction cycle.
  • WEB-2086 prevented the negative inotropic effects of PAF.

Conclusions:

  • PAF exerts a direct, receptor-mediated negative inotropic effect on adult cardiomyocytes.
  • WEB-2086 antagonizes PAF's negative inotropic action and possesses independent positive inotropic properties.
  • Isolated cardiomyocyte preparations are crucial for dissecting direct cellular responses to mediators like PAF.