Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A justification for high resolution hematocrit measurement

M E Kelly1, M J Luetkemeier, G M Pantalos

  • 1University of Utah, Department of Exercise and Sport Science, Salt Lake City 84112.

Medicine and Science in Sports and Exercise
|May 1, 1994
PubMed
Summary

A new digital caliper technique (DC) offers a highly precise and accurate method for measuring hematocrit (HCT) from microhematocrit tubes. This advancement allows for more reliable detection of subtle changes in plasma volume in research settings.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An analysis of applications and acceptances to the National Specialist Training Programme in General Practice Ireland from 2021-2024.

Irish medical journal·2025
Same author

Retraction.

Irish medical journal·2025
Same author

RETRACTED: An analysis of applications and acceptances to the National Specialist Training Programme in General Practice Ireland from 2021-2024.

Irish medical journal·2025
Same author

A comparison of surgical techniques for perineal wound closure following perineal excision: a systematic review and network meta-analysis.

Techniques in coloproctology·2023
Same author

Metastatic Lobular Breast Carcinoma of the Urinary Bladder After Eight Years in Remission.

Irish medical journal·2023
Same author

Outcomes of endoscopic full thickness resection in the colon rectum at an Italian tertiary center.

Techniques in coloproctology·2023

Area of Science:

  • Clinical Chemistry
  • Hematology
  • Biomedical Engineering

Background:

  • Accurate hematocrit (HCT) measurement is crucial for assessing plasma volume changes.
  • Conventional methods like the microhematocrit centrifuge (MC) and Coulter Counter (CC) have limitations in precision.
  • A need exists for a more reliable and accurate HCT measurement technique.

Purpose of the Study:

  • To evaluate the feasibility of using a digital caliper and microscope for HCT measurement.
  • To compare the reliability and validity of the digital caliper technique (DC) against MC and CC methods.

Main Methods:

  • Hematocrit was measured using three methods: microhematocrit centrifuge (MC), Coulter Counter (CC), and digital caliper (DC).
  • Reliability was assessed by the standard error of the measurement (SEm) from triplicate readings.

Related Experiment Videos

  • Validity was determined by comparing HCT values with densitometry measurements.

    • Main Results:

    • The digital caliper technique (DC) exhibited the lowest standard error of the measurement (SEm = 0.1491), indicating high reliability.
    • Average HCT values for MC, CC, DC, and densitometry were 42.3, 42.4, 43.1, and 43.7, respectively.
    • Only the DC method's HCT values were not significantly different from densitometry (P > 0.05), while MC and CC values were significantly lower (P < 0.01).

    Conclusions:

    • Accurate and highly precise hematocrit measurements are achievable using a digital caliper and microscope.
    • The DC method offers superior reliability and validity compared to conventional MC and CC methods.
    • This technique enhances the ability to accurately measure small changes in plasma volume, benefiting research applications.