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[The coagulase test: considerations. II note (author's transl)]

F Chiarini, M Del Piano, R Nicosia

    Annali Sclavo; Rivista Di Microbiologia E Di Immunologia
    |November 1, 1976
    PubMed
    Summary

    This study investigated Staphylococcus strains from patient samples, analyzing coagulase and clumping factor activity using various animal plasmas and anticoagulants. Results offer insights into Staphylococcus virulence factors and diagnostic testing methods.

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    Area of Science:

    • Microbiology
    • Bacteriology

    Context:

    • Staphylococcus species are significant human pathogens.
    • Accurate identification of Staphylococcus strains is crucial for effective treatment.
    • Coagulase and clumping factor are key virulence factors in Staphylococcus.

    Purpose:

    • To evaluate the activity of free coagulase and clumping factor in Staphylococcus strains.
    • To compare the efficacy of different plasma types (human, pig, rabbit) in detecting these factors.
    • To assess the influence of anticoagulants (citrate, heparin) on test results.

    Summary:

    • Twenty-two Staphylococcus strains from pathological specimens were tested for free coagulase and clumping factor.
    • Assays were performed using human, pig, and rabbit plasma with citrate and heparin anticoagulants.

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  • The study analyzed test results individually and in relation to each other.

    • Impact:

    • Provides data on the variability of coagulase and clumping factor expression in clinical Staphylococcus isolates.
    • Informs the selection of optimal reagents and methods for Staphylococcus identification in diagnostic laboratories.
    • Contributes to understanding Staphylococcus pathogenicity and developing improved diagnostic strategies.