Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Replication in Prokaryotes

Replication in Prokaryotes

Replication in Prokaryotes

Replication in Prokaryotes

Replication in Prokaryotes

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...

Coordination of Gene Expression Processes in Bacteria

Coordination of Gene Expression Processes in Bacteria

The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...

Stringent Response in E. coli

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...

Inhibitors of Bacterial DNA Synthesis

Inhibitors of Bacterial DNA Synthesis

Bacterial pathogens depend on precise and efficient DNA replication to sustain infection. Two type II topoisomerases—DNA gyrase and topoisomerase IV—are critical to this process, as they resolve DNA supercoiling and unlink chromosomes during replication. Fluoroquinolones, synthetic derivatives of quinolones, exploit this mechanism by stabilizing the transient DNA–enzyme cleavage complex, preventing strand religation, and causing lethal double-strand breaks. These antibiotics are selectively...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Using available <i>in vitro</i> metabolite identification and time course kinetics for β-chloroprene and its metabolite, (1-chloroethenyl) oxirane, to include reactive oxidative metabolites and glutathione depletion in a PBPK model for β-chloroprene.

Frontiers in pharmacology·2023

Same author

Saline Transfusion, Hemorrhage and Shock.

Atlanta journal-record of medicine·2022

Same author

Acute Surgical Conditions of the Abdomen Often Mistaken for Appendicitis.

Atlanta journal-record of medicine·2022

Same author

The Gall Bladder.

Atlanta journal-record of medicine·2022

Same author

Scopolamine and Morphine in Labor-A Clinical Study of Twenty-One Cases.

Atlanta journal-record of medicine·2022

Same author

A Clinical Lecture on Gastric and Duodenal Ulcer.

Atlanta journal-record of medicine·2022

Search research articles

Related Experiment Video

Updated: Jul 15, 2026

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

SeqA: a negative modulator of replication initiation in E. coli

M Lu¹, J L Campbell, E Boye

¹Department of Biochemistry and Molecular Biology Harvard University, Cambridge, Massachusetts 02138.

|May 6, 1994

Summary

The SeqA gene controls bacterial DNA replication initiation by preventing premature starts. This gene acts as a negative regulator, balancing positive factors for precise cell cycle control.

More Related Videos

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Published on: September 11, 2017

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Related Experiment Videos

Last Updated: Jul 15, 2026

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Published on: September 11, 2017

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Area of Science:

Molecular Biology
Microbiology
Bacterial Cell Cycle Regulation

Background:

Bacterial DNA replication initiates at a unique origin, oriC.
In rapidly growing cells, multiple oriC copies exist and initiate synchronously once per cell cycle.
A sequestration process prevents secondary initiations by targeting hemimethylated GATC sites on newly replicated origins.

Purpose of the Study:

To identify the gene responsible for the sequestration process in E. coli.
To investigate the role of the identified gene in regulating DNA replication initiation.
To understand the balance of positive and negative regulatory elements in replication control.

Main Methods:

Genetic screening to identify genes required for sequestration.
In vivo assays to assess the function of the identified gene (seqA) in replication initiation.
Analysis of seqA's interaction with hemimethylated origins and initiation factors.

Main Results:

Identification of the gene seqA as essential for the sequestration of hemimethylated origins.
Demonstration that seqA acts as a negative modulator of primary replication initiation.
Observation that seqA's function contrasts with previously identified positive initiation factors.

Conclusions:

Precise control of bacterial DNA replication initiation involves a balance between positive and negative regulatory elements.
The SeqA protein plays a crucial role as a negative regulator, preventing premature replication.
SeqA may function as a cooperativity factor, mediating interactions among replication initiation components.