Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Probing immune functions in RAG-deficient mice

J Chen1, Y Shinkai, F Young

  • 1Howard Hughes Medical Institute, Children's Hospital, Boston.

Current Opinion in Immunology
|April 1, 1994
PubMed
Summary

Mice lacking recombination activating gene (RAG)-1 or RAG-2 cannot initiate V(D)J recombination, halting lymphocyte differentiation. Introducing functional genes bypasses this block, enabling analysis of lymphocyte development and gene function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intraoperative cell salvage.

BJA education·2021
Same author

Towards remote healthcare monitoring using accessible IoT technology: state-of-the-art, insights and experimental design.

Biomedical engineering online·2020
Same author

[Investing for children's early development: the role of maternal and child health practitioners].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2018
Same author

Papilloedema secondary to acute purulent sinusitis.

Journal of paediatrics and child health·2017
Same author

Assessment of serological tests for diagnosis of bovine tuberculosis.

The Veterinary record·2017
Same author

Pathogen and Particle Associations in Wastewater: Significance and Implications for Treatment and Disinfection Processes.

Advances in applied microbiology·2016

Area of Science:

  • Immunology
  • Developmental Biology
  • Genetics

Background:

  • Recombination activating gene (RAG)-1 and RAG-2 are essential for V(D)J recombination in developing lymphocytes.
  • Inactivation of RAG genes leads to a severe block in lymphocyte differentiation at an early stage.

Purpose of the Study:

  • To investigate methods for overcoming the V(D)J recombination block in RAG-deficient lymphocytes.
  • To develop new strategies for analyzing the control of lymphocyte differentiation and gene function.

Main Methods:

  • Introduction of functionally assembled antigen-receptor genes into RAG-deficient mice.
  • Generation of somatic chimeric mice by injecting mutant embryonic stem cells into RAG-2-deficient blastocysts.

Main Results:

Related Experiment Videos

  • Bypassing the V(D)J recombination block promotes lymphocyte differentiation to various stages.
  • Somatic chimeric mice provide a powerful model for assessing gene roles in lymphocyte development.

Conclusions:

  • Functional gene introduction is a viable strategy to study lymphocyte differentiation in the absence of V(D)J recombination.
  • Somatic chimera generation offers a novel approach for dissecting gene regulatory roles in lymphocyte development and function.