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Related Experiment Videos

Modeling of high cell density fed batch cultivation

L Andersson1, L Strandberg, L Häggström

  • 1Department of Biochemistry and Biotechnology, Royal Institute of Technology, Stockholm, Sweden.

FEMS Microbiology Reviews
|May 1, 1994
PubMed
Summary

Fed batch cultures of Escherichia coli showed that both substrate feed rate and salt concentration significantly impact biomass yield. High salt levels and feed rates affect bacterial energetics and growth kinetics.

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Area of Science:

  • Microbiology
  • Biotechnology
  • Biochemical Engineering

Background:

  • Fed batch cultures are widely used for microbial biomass production.
  • Understanding growth kinetics under stress is crucial for optimizing bioprocesses.
  • Escherichia coli is a model organism for studying bacterial physiology.

Purpose of the Study:

  • To investigate the effects of osmotic stress and varying substrate feed rates on Escherichia coli growth kinetics.
  • To evaluate the applicability of a linear substrate consumption model under different conditions.
  • To determine factors limiting biomass production in fed batch cultures.

Main Methods:

  • Cultivation of Escherichia coli in carbon- and energy-limited fed batch cultures.
  • Introduction of osmotic stress using 0.5 M NaCl.

Related Experiment Videos

  • Modeling bacterial growth using a linear equation for substrate consumption.
  • Monitoring growth kinetics, substrate uptake, and viability.

    • Main Results:

    • A linear substrate consumption model adequately described early-phase growth (20 h) in unstressed cultures.
    • Osmotic stress (0.5 M NaCl) significantly altered bacterial energetics beyond a simple increase in maintenance requirement.
    • Later growth phases exhibited decreased viability and a lower limit for specific sugar uptake rate across all cultures.
    • Total obtainable biomass was strongly influenced by substrate feed rate and medium ionic strength.

    Conclusions:

    • Substrate feed rate and ionic strength are critical determinants of total biomass yield in fed batch cultures.
    • Osmotic stress profoundly impacts bacterial energetics and growth, necessitating more complex modeling.
    • Bacterial viability decline and substrate uptake limitations mark the later stages of fed batch cultivation.