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In vitro biocompatibility evaluation of a heparinizable material (PUPA), based on polyurethane and poly(amido-amine)
A Albanese1, R Barbucci, J Belleville
1CRISMA, Nuovo Policlinico Le Scotte, Siena, Italy.
Biomaterials
|January 1, 1994
Summary
This study evaluated a new polyurethane and poly(amido-amine) (PUPA) material for biocompatibility. Gamma-ray sterilization proved suitable, showing no toxic effects on the heparinized or non-heparinized PUPA material.
Area of Science:
- Biomaterials Science
- Polymer Chemistry
- Biocompatibility Testing
Background:
- Developing advanced biomaterials is crucial for medical devices.
- Heparinizable materials aim to improve blood compatibility.
- Polyurethane and poly(amido-amine) (PUPA) is a novel material composition.
Purpose of the Study:
- To evaluate the biocompatibility of a new heparinizable PUPA material.
- To assess heparin quantity, release, and interaction with blood components.
- To determine the impact of sterilization on PUPA cytocompatibility.
Main Methods:
- Quantification of heparin using radiolabeled heparin and biological assays.
- In vitro assessment of heparin release into plasma.
- Evaluation of PUPA's interaction with cellular and plasmatic blood components.
- Cytocompatibility testing after gamma-ray sterilization.
Main Results:
- The PUPA material demonstrated good biocompatibility in both heparinized and non-heparinized states.
- Heparin quantification and release profiles were established.
- PUPA showed favorable interactions with blood components.
- Gamma-ray sterilization did not induce a toxic response in PUPA.
Conclusions:
- The new heparinizable PUPA material exhibits promising biocompatibility.
- Gamma-ray sterilization is a viable method for this material.
- PUPA is a suitable candidate for applications requiring enhanced blood compatibility.