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Real time measurement of gene expression in living endocrine cells
L S Frawley1, W J Faught, J Nicholson
1Dept. of Cell Biology and Anatomy, Medical University of South Carolina, Charleston 29425.
Endocrinology
|July 1, 1994
Abstract:
The mechanisms underlying hormonal gene expression are complex, and key regulatory events are often temporally separated. Our understanding of these dynamic processes has been hindered in part by the lack of a method suitable for making dynamic measurements of gene expression. In an attempt to develop such a system, we modified conventional luciferase reporter technology to enable real time measurement of gene expression in living endocrine cells.