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Disruption of endothelial barrier function: relationship to fluidity of membrane extracellular lamella
A Alvarado1, D A Butterfield, B Hennig
1Department of Nutrition and Food Science, University of Kentucky, Lexington 40506-0054.
Abstract:
1. Endothelial cells were cultured in tissue culture flasks or on microcarrier beads and labeled with a lipid specific spin-label. 2. Exposure of endothelial cells to benzyl alcohol caused a dose- and time-dependent increase in membrane fluidity using electron spin resonance (ESR). Maximum fluidity was reached after a 5-min exposure to 100 mM benzyl alcohol. 3. Albumin permeability across endothelial cells cultured on micropore filters was used as an indication of endothelial monolayer integrity. 4. A significant increase in permeability occurred with 50 mM benzyl alcohol. Maximal albumin permeability was reached after a 5-min exposure to 100 mM benzyl alcohol.