Fluorescence in situ hybridization: a new tool for the pathologist
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Updated: Aug 17, 2026
12:15
Double Fluorescence in situ Hybridization in Fresh Brain Sections
Published on: August 14, 2010
1Oncor, Inc., Gaithersburg, MD 20877.
Human Pathology
|June 1, 1994
Abstract:
DNA in situ hybridization methods permit investigations of genetic alterations within the context of cell morphology and tissue architecture and are of particular importance in tumor pathology. Detection of specific chromosome aberrations may be a critical adjunct to diagnosis or prognosis when morphology is ambiguous. This approach will lead to better understanding of heterogeneity in tumors and to better definition of premalignant lesions.
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02:07FISH - Fluorescent In-situ Hybridization
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
02:31In-situ Hybridization
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
01:12Immunofluorescence Microscopy
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...
The...