Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Iron overload in beta 2-microglobulin-deficient mice

M de Sousa1, R Reimão, R Lacerda

  • 1Abel Salazar Institute for the Biomedical Sciences, Oporto, Portugal.

Immunology Letters
|February 1, 1994
PubMed
Summary

Mice with a mutated beta 2 microglobulin (B2M) gene showed progressive liver iron overload, similar to human hemochromatosis. Homozygous mice had significantly higher hepatic iron levels than heterozygotes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Robotic endoscopic transforaminal lumbar interbody fusion: A single institution case series.

World neurosurgery: X·2024
Same author

Iron triggers the early stages of cartilage degeneration <i>in vitro</i>: The role of articular chondrocytes.

Osteoarthritis and cartilage open·2022
Same author

Combining jobs and motherhood: is it worse when raising children alone?

Journal of public health (Oxford, England)·2021
Same author

The "Football is Medicine" platform-scientific evidence, large-scale implementation of evidence-based concepts and future perspectives.

Scandinavian journal of medicine & science in sports·2018
Same author

Expression of iron-related proteins in feline and canine mammary gland reveals unexpected accumulation of iron.

Biotechnic & histochemistry : official publication of the Biological Stain Commission·2017
Same author

Tissue iron quantification in chronic liver diseases using MRI shows a relationship between iron accumulation in liver, spleen, and bone marrow.

Clinical radiology·2017

Area of Science:

  • Biochemistry
  • Genetics
  • Pathology

Background:

  • Beta 2 microglobulin (B2M) plays a role in immune function and protein complex formation.
  • Genetic mutations in B2M can lead to various physiological alterations.
  • Iron overload disorders, such as hemochromatosis, have significant health implications.

Purpose of the Study:

  • To investigate the impact of beta 2 microglobulin gene mutations on iron distribution in mice.
  • To compare iron levels in homozygous (beta 2m-/-) and heterozygous (beta 2m+/-) mice.
  • To assess the potential development of hepatic iron overload resembling human hemochromatosis.

Main Methods:

  • Comparative histological analysis of tissue iron distribution.
  • Quantitative analysis using flame atomic absorption spectroscopy for total iron content.

Related Experiment Videos

  • Study involved mice of different ages with varying beta 2 microglobulin gene statuses.

    • Main Results:

    • Progressive hepatic iron overload was observed exclusively in mice homozygous for the mutated beta 2m gene.
    • The observed iron overload was histologically indistinguishable from human hemochromatosis.
    • Homozygous mice exhibited significantly higher mean hepatic iron levels (1583 +/- 423 µg/g dry weight) compared to heterozygotes (468 +/- 174 µg/g dry weight).

    Conclusions:

    • Homozygosity for the mutated beta 2m gene leads to progressive hepatic iron overload in mice.
    • This mouse model mimics key aspects of human hemochromatosis, offering a valuable research tool.
    • B2M deficiency is strongly implicated in the pathogenesis of iron accumulation in the liver.