Detection of liver fibrosis with magnetic cross-relaxation

The utility of MRI using magnetization transfer (MT) enhanced pulse sequences to diagnose hepatic cirrhosis in a rat model was investigated. Hepatic T1 was measured with and without MT off-resonance RF pulses in 17 treated and six control rats. The livers were evaluated histologically, and the hydroxyproline content quantitatively measured. We did not find a statistically significant linear correlation between the MR relaxation times and the degree of tissue injury. However, the MR measurements performed with MT were superior to those without differentiating the treated and control groups. Specifically, the T1 times were 695 +/- 76 ms for the treated group, versus 748 +/- 61 ms in the controls; P = 0.095. The T1sat times were also lower in the treated group, with statistical significance: 367 +/- 51 ms versus 421 +/- 38 ms, P = 0.016. Finally, the change in the relaxation rates (the inverse of the relaxation times) with and without saturation were 1.31 +/- 0.22 s-1 (treated group) versus 1.05 +/- 0.12 s-1 (controls), which differed significantly, P = 0.001.