Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

No limits on restriction

G Riddihough

    Nature
    |July 7, 1994
    PubMed
    Summary
    This summary is machine-generated.

    The structure of the PvuII endonuclease enzyme, with and without its DNA recognition site, reveals insights into how this enzyme binds and interacts with DNA.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The evolution of epigenetics.

    Science (New York, N.Y.)·2001
    Same author

    Setting our sights on the stars.

    Proceedings of the National Academy of Sciences of the United States of America·1999
    Same author

    A never ending frontier.

    Nature structural biology·1997
    Same author

    Picture story. A fork in the works.

    Nature structural biology·1996
    Same author

    Picture story. Jellyroll boat.

    Nature structural biology·1996
    Same author

    DNA ligase shows restraint.

    Nature structural biology·1996
    Same journal

    Daily briefing: How cooperation built the world.

    Nature·2026
    Same journal

    Deep-sea oddities and boatloads of other new species - June's best science images.

    Nature·2026
    Same journal

    From cloning to gene-editing: the enduring legacy of Dolly the sheep.

    Nature·2026
    Same journal

    Time to give hydration breaks the red card? What science says about keeping cool.

    Nature·2026
    Same journal

    Universities are relying on AI-detection software to catch cheating. How well do the programs work?

    Nature·2026
    Same journal

    Daily briefing: 'Cyborg' cockroaches breathe underwater with printed suit.

    Nature·2026
    See all related articles

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Structural Biology

    Background:

    • Endonucleases are enzymes that play crucial roles in DNA metabolism, including replication, repair, and recombination.
    • Understanding the structural basis of endonuclease-DNA interactions is fundamental to elucidating their biological functions.

    Purpose of the Study:

    • To elucidate the structural mechanisms underlying the recognition and binding of DNA by the endonuclease R.PvuII.
    • To provide insights into the molecular interactions between R.PvuII and its specific DNA recognition sequence.

    Main Methods:

    • X-ray crystallography was employed to determine the three-dimensional structures of R.PvuII.
    • Structural analysis was performed on the enzyme both in its apo form (without DNA) and in complex with its cognate DNA recognition site.

    Related Experiment Videos

    Main Results:

    • The study determined the crystal structures of R.PvuII in two distinct states: apo and DNA-bound.
    • Structural comparisons revealed conformational changes in R.PvuII upon binding to its DNA recognition site, highlighting key amino acid residues involved in DNA interaction.
    • The observed structural features provide a model for how R.PvuII approaches and binds to its target DNA sequence.

    Conclusions:

    • The structural data elucidates the mechanism of DNA recognition and binding by R.PvuII.
    • The findings contribute to a deeper understanding of endonuclease function and DNA-protein interactions at a molecular level.
    • This work provides a structural basis for future studies on enzyme engineering and drug development targeting DNA-modifying enzymes.